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ai 2000  (Vector Laboratories)


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    Structured Review

    Vector Laboratories ai 2000
    Ai 2000, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 2161 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/AI-2000-1%2E5/Unconjugated+Horse+Anti-Mouse+IgG+Antibody/pm40723176-67-0-2
    Average 95 stars, based on 2161 article reviews
    ai 2000 - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Membrane:

    Article Title: Delta opioid receptors affect acoustic features of song during vocal learning in zebra finches.
    Article Snippet: For detecting PSD-95 and Synaptotagmin proteins, membranes were incubated with anti-PSD-95, (ab9708, Abcam, USA; 1:2000 dilution; made in rabbit) and anti-synaptotagmin (MAB5200, MERCK Millipore; USA; 1:1000 dilution; made in mouse), respectively, also for 16 h at 4 °C. .. This was followed by incubating the membrane in a solution containing the alkaline phosphatase-labelled anti-rabbit antibody (1:1000; raised in goat, PI-1000; Vector Laboratories, Burlingame, CA; for DARPP-32 and PSD-95) and the alkaline phosphatase-labelled anti-mouse antibody (1:1000; raised in horse, Vector Laboratories, Burlingame, CA) for 1 h. The ECL chromogenic method was then used to detect bands specific for DARPP-32. .. Major song control nuclei (LMAN, Area X, HVC and RA) in control and naltrindole-treated birds (n = 9 sets) were outlined in their entirety, using the Stereoinvestigator software (MBF Bioscience).

    Binding Assay:

    Article Title: PET imaging of TREM2 in amyloid-beta induced neuroinflammation
    Article Snippet: Horseradish peroxidase (HRP) conjugated streptavidin (Mabtech, 3310-9-1000) was then added at a 1:2000 dilution for 1 h at RT, on a shaker. .. To compare binding affinity before and after radiolabeling, the plate was coated with the following proteins and antibodies, diluted in PBS: anti-mouse IgG (0.5 μg/mL, VECTOR, AI-2000-1.5), anti-human IgG (0.5 μg/mL, Mabtech, MT91/145), StrepMAB-Classic (0.5 μg/mL, Iba Life Sciences, 2–1507-001), murine TfR protein (0.5 μg/mL, in-house produced), and murine TREM2 protein (5 μg/mL, SinoBiological, 158–50149-M02H-100). ..

    Article Title: PET imaging of TREM2 in amyloid-beta induced neuroinflammation.
    Article Snippet: Horseradish peroxidase (HRP) conjugated streptavidin (Mabtech, 3310-9-1000) was then added at a 1:2000 dilution for 1 h at RT, on a shaker. .. To compare binding affinity before and after radiolabeling, the plate was coated with the following proteins and antibodies, diluted in PBS: anti-mouse IgG (0.5 μg/ mL, VECTOR, AI-2000-1.5), anti-human IgG (0.5 μg/ mL, Mabtech, MT91/145), StrepMAB-Classic (0.5 μg/ mL, Iba Life Sciences, 2–1507-001), murine TfR protein (0.5 μg/mL, in-house produced), and murine TREM2 protein (5 μg/mL, SinoBiological, 158–50149-M02H-100). ..

    Radioactivity:

    Article Title: PET imaging of TREM2 in amyloid-beta induced neuroinflammation
    Article Snippet: Horseradish peroxidase (HRP) conjugated streptavidin (Mabtech, 3310-9-1000) was then added at a 1:2000 dilution for 1 h at RT, on a shaker. .. To compare binding affinity before and after radiolabeling, the plate was coated with the following proteins and antibodies, diluted in PBS: anti-mouse IgG (0.5 μg/mL, VECTOR, AI-2000-1.5), anti-human IgG (0.5 μg/mL, Mabtech, MT91/145), StrepMAB-Classic (0.5 μg/mL, Iba Life Sciences, 2–1507-001), murine TfR protein (0.5 μg/mL, in-house produced), and murine TREM2 protein (5 μg/mL, SinoBiological, 158–50149-M02H-100). ..

    Article Title: PET imaging of TREM2 in amyloid-beta induced neuroinflammation.
    Article Snippet: Horseradish peroxidase (HRP) conjugated streptavidin (Mabtech, 3310-9-1000) was then added at a 1:2000 dilution for 1 h at RT, on a shaker. .. To compare binding affinity before and after radiolabeling, the plate was coated with the following proteins and antibodies, diluted in PBS: anti-mouse IgG (0.5 μg/ mL, VECTOR, AI-2000-1.5), anti-human IgG (0.5 μg/ mL, Mabtech, MT91/145), StrepMAB-Classic (0.5 μg/ mL, Iba Life Sciences, 2–1507-001), murine TfR protein (0.5 μg/mL, in-house produced), and murine TREM2 protein (5 μg/mL, SinoBiological, 158–50149-M02H-100). ..

    Produced:

    Article Title: PET imaging of TREM2 in amyloid-beta induced neuroinflammation
    Article Snippet: Horseradish peroxidase (HRP) conjugated streptavidin (Mabtech, 3310-9-1000) was then added at a 1:2000 dilution for 1 h at RT, on a shaker. .. To compare binding affinity before and after radiolabeling, the plate was coated with the following proteins and antibodies, diluted in PBS: anti-mouse IgG (0.5 μg/mL, VECTOR, AI-2000-1.5), anti-human IgG (0.5 μg/mL, Mabtech, MT91/145), StrepMAB-Classic (0.5 μg/mL, Iba Life Sciences, 2–1507-001), murine TfR protein (0.5 μg/mL, in-house produced), and murine TREM2 protein (5 μg/mL, SinoBiological, 158–50149-M02H-100). ..

    Article Title: PET imaging of TREM2 in amyloid-beta induced neuroinflammation.
    Article Snippet: Horseradish peroxidase (HRP) conjugated streptavidin (Mabtech, 3310-9-1000) was then added at a 1:2000 dilution for 1 h at RT, on a shaker. .. To compare binding affinity before and after radiolabeling, the plate was coated with the following proteins and antibodies, diluted in PBS: anti-mouse IgG (0.5 μg/ mL, VECTOR, AI-2000-1.5), anti-human IgG (0.5 μg/ mL, Mabtech, MT91/145), StrepMAB-Classic (0.5 μg/ mL, Iba Life Sciences, 2–1507-001), murine TfR protein (0.5 μg/mL, in-house produced), and murine TREM2 protein (5 μg/mL, SinoBiological, 158–50149-M02H-100). ..

    Incubation:

    Article Title: The mechanism of Micafungin action to Pteropine orthoreovirus infection in human cell line
    Article Snippet: .. After incubation, the wells were washed three times with PBS-T, and Horse Anti-Mouse IgG Antibody (H+L), Peroxidase (Vector Laboratories, Newark, CA), diluted 1:1000, was added to each well and incubated for 1 hour. ..

    other:

    Article Title: First generation vanadium-based PTEN inhibitors: Comparative study in vitro and in vivo and identification of a novel mechanism of action.
    Article Snippet: PTEN, a tumor suppressor phosphatase, regulates cellular functions by antagonizing the growth promoting PI3K/ Akt/mTOR pathway through the dephosphorylation of the second messenger PIP3.. Many preclinical cellular and animal studies have used PTEN inhibitors to highlight specific disease contexts where acute activation of PI3K/ Akt/mTOR pathway might offer therapeutic advantages.. In the present study we have re-evaluated first-generation PTEN inhibitors, including established bisperoxo-vanadium(V) complexes (bpVs).



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